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Michaelis–Menten characterization of MINA53 and NO66 for the 2OG cosubstrate and ribosomal peptide substrates. (A) Determination of the KM for 2OG with MINA53 (Met26-Val464) and (B) NO66 (Ser183-Asn641). Values are means ± 95% confidence, n = 4. (C) Determination of KM for the peptide substrate with MINA53 (Met1-Val464) and (D) NO66 (Gln116-Asn641). Values are means ± 95% confidence, n = 3. See Table S4 for assay details.
