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. 2021 Nov;9(22):1672. doi: 10.21037/atm-21-5369

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Protein localization of wild-type and mutant of DSPP gene. Confocal microscopy shows intracellular localization of the GFP-tagged WT and mutant (p. Val18Gly) DSP in HEK293T cells. Anti-CANX antibody was used for ER staining, and a GM130 antibody was used for Golgi staining. Nuclei were stained with DAPI. (A,C) Wild-type DSP was predominantly found in the Golgi apparatus. (B,D) The p. Val18Gly mutant DSP was found predominantly in the ER, even though a portion was localized in the Golgi apparatus. GFP, green ﬂuorescent protein; WT, wild-type; DSPP, dentin sialophosphoprotein; DSP, dentin sialoprotein; CANX, anti-calnexin; GM130, anti-Golgi matrix protein; ER, endoplasmic reticulum; DAPI, 4',6-diamidino-2-phenylindole.