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. 2021 Nov;9(22):1694. doi: 10.21037/atm-21-5752

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Sal inhibited OGD/R-induced apoptosis of BV2 cells. (A) Apoptosis of BV2 cells was detected by flow cytometry; (B) percentages of apoptotic cells were quantitatively analyzed (n=4); (C) representative fluorescent images of TUNEL staining (green) and DAPI (blue) (scale bar =100 µm); (D) quantitative analysis of TUNEL staining (n=5); (E) Western blot analysis (n=3) of protein expression levels of cleaved caspase-3, Bax, and Bcl-2. Data are expressed as mean ± SD. ***P<0.001 compared with control group; ^#P<0.05, ^##P<0.01, ^###P<0.001 compared with OGD/R group. OGD, oxygen and glucose deprivation; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling assay; SD, standard deviation.