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. 2021 Nov;9(22):1701. doi: 10.21037/atm-21-5975

Table 1. Gene primer sequences.

Gene Forward primer Reverse primer
Bak 5'-TGAGTACTTCACCAAGATTCA-3' 5'-AGTCAGGCCATGCTGGTAGAC-3'
FBP1 5'-GGTGGACAAGGATGTGAAGATA-3' 5'-GGGAACTTCTTCCTCTGGATG-3'
PKM2 5'-CTTGCAATTATTTGAGGAACTCCGC-3' 5'-CACGGTACAGGTGGGCCTGAC-3'
LDHA 5'-AAGCGGTTGCAATCTGGATTCAG-3' 5'-GGTGAACTCCCAGCCTTTCC-3'
GLUT1 5'-GATTGGCTCCTTCTCTGTGG-3' 5'-TCAAAGGACTTGCCCAGTTT-3'
PEPCK1 5'-GCTCTGAGGAGGAGAATGG-3' 5'-TGCTCTTGGGTGACGATAAC-3'
PFK 5'-GGTGGACGGAGGAGATAACA-3' 5'-CAGTCAGGGAACCATCACCT-3'
β-actin 5'-CAGCCTTCCTTCCTGGGCATG-3' 5'-ATTGTGCTGGGTGCCAGGGCAG-3'

Bak, Bcl-2-antagonist/killer (Bak); FBP1, fructose-1,6-bisphosphatase 1; PKM2, pyruvate kinase M2; LDHA, lactate dehydrogenase A; GLUT1, glucose transporter 1; PEPCK1, phosphoenolpyruvate carboxykinase 1; PFK, phosphofructokinase.