Table 1. Gene primer sequences.
Gene | Forward primer | Reverse primer |
---|---|---|
Bak | 5'-TGAGTACTTCACCAAGATTCA-3' | 5'-AGTCAGGCCATGCTGGTAGAC-3' |
FBP1 | 5'-GGTGGACAAGGATGTGAAGATA-3' | 5'-GGGAACTTCTTCCTCTGGATG-3' |
PKM2 | 5'-CTTGCAATTATTTGAGGAACTCCGC-3' | 5'-CACGGTACAGGTGGGCCTGAC-3' |
LDHA | 5'-AAGCGGTTGCAATCTGGATTCAG-3' | 5'-GGTGAACTCCCAGCCTTTCC-3' |
GLUT1 | 5'-GATTGGCTCCTTCTCTGTGG-3' | 5'-TCAAAGGACTTGCCCAGTTT-3' |
PEPCK1 | 5'-GCTCTGAGGAGGAGAATGG-3' | 5'-TGCTCTTGGGTGACGATAAC-3' |
PFK | 5'-GGTGGACGGAGGAGATAACA-3' | 5'-CAGTCAGGGAACCATCACCT-3' |
β-actin | 5'-CAGCCTTCCTTCCTGGGCATG-3' | 5'-ATTGTGCTGGGTGCCAGGGCAG-3' |
Bak, Bcl-2-antagonist/killer (Bak); FBP1, fructose-1,6-bisphosphatase 1; PKM2, pyruvate kinase M2; LDHA, lactate dehydrogenase A; GLUT1, glucose transporter 1; PEPCK1, phosphoenolpyruvate carboxykinase 1; PFK, phosphofructokinase.