FIG. 2.
Expression of AGP1 and PTR2 in wild-type (WT) and ssy5 null mutant strains. Strains PLY126 (wild type) and HKY77 (ssy5Δ2) were grown in SD to an OD600 of 0.8, and total RNA was isolated 45 min after the addition of water (lanes 1 and 3) or 0.15 mM leucine (lanes 2 and 4). Expression levels of AGP1 (A) and PTR2 (B) were determined by Northern analysis and quantitated by phosphorimaging. The levels of actin (ACT1) transcript were used to standardize quantitations (lower panels). The relative expression levels were normalized to the expression levels in the uninduced wild-type strain.