TABLE 2.
Plasmids and oligonucleotides used in this study
| Plasmid or oligonucleotide | Description or sequence | Reference |
|---|---|---|
| Plasmids | ||
| pPL193 | 5-kb EcoRI fragment containing PTR3 in pRS316 | 34 |
| pPL341 | ptr3Δ14::hisG-URA3-kanr-hisG in pUC118 (ΔB-ΔH) | 34 |
| pPL356 | 3.9-kb SpeI-ClaI fragment containing SSY1 in pRS316 | 34 |
| pPL496 | 7.8-kb Sau3A fragment containing SSY5 in pRS202 | This work |
| pHK003 | pPL356 with all XbaI sites removed | 34 |
| pHK010 | SSY1-HA1 in pRS316 | 34 |
| pHK012 | SSY1 in pRS202 | This work |
| pHK018 | PTR3-HA1 in pRS316 | 34 |
| pHK026 | PTR3 in pRS202 | This work |
| pHK031 | ssy1Δ12::hisG-URA3-kanr-hisG in pBluescript | This work |
| pHK035 | 4.9-kb BstXI-BamHI fragment containing SSY5 in pRS316 | This work |
| pHK036 | Same as pHK035, except SSY5 fragment inserted in opposite orientation | This work |
| pHK037 | SSY5 in pRS202 | This work |
| pHK038 | SSY1-NT(1–206) in pRS316 | This work |
| pHK039 | SSY1-NT(1–206) in pRS202 | This work |
| pHK040 | pPL193 with BamHI site in MCS removed | This work |
| pHK041 | pHK036 with BamHI site in MCS removed | This work |
| pHK042 | pHK040 with BamHI site (b) introduced after ATG in PTR3 | This work |
| pHK043 | pHK041 with BamHI site (b) introduced after ATG in SSY5 | This work |
| pHK044 | PTR3 in pSOS | This work |
| pHK045 | SSY5 in pSOS | This work |
| pHK047 | pHK041 with BamHI site (a) introduced after the ATG of SSY5 ORF | This work |
| pHK048 | SSY5-c-myc in pRS316 | This work |
| pHK049 | ssy5Δ1::hisG-URA3-kanr-hisG in pRS316 | This work |
| pHK050 | SSY5 in pAD40 | This work |
| Oligonucleotides | ||
| POL95-039 | 5′-GATTGAGTTGAATTCTTCTACCACTAC-3′ | |
| POL96-021 | 5′-CCTGGCTGATTTCTAGATAGGGTTATATG-3′ | |
| POL99-026 | 5′-GATATACCGATGGGATCCGTCAGATTTTTTGG-3′ | |
| POL00-004 | 5′-GATATACCGATGGGGATCCTCAGATTTTTTGG-3′ | |
| POL00-005 | 5′-TAGGCGTTCATGGGGATCCACTCTCATAG-3′ |