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ROS generation in HeLa cells using DCFDA. HeLa cells were incubated with 4 (25 μM) and DCFDA (10 μM), and fluorescence was monitored before and after 5 min irradiations with a 525 nm green lamp (23.60 mW/cm2). Green fluorescence indicates the production of ROS in cells. 20× magnification, scale bar = 50 μm.
