Figure 2.

Progesterone increases expression of α-SMA and TGF-β in fibrotic lung tissue. (A) 5 µm sections of paraffin embedded left lungs were taken. Slides incubated with anti α-SMA antibody, stained with Diaminobenzidine and counter - stained with Haematoxylin. Original magnification = 200X. Scale = 100 μm (B) Quantitative expression of α-SMA in lung tissues. (C) TGF-β in lung tissues. Results are represented as mean ± SEM, n= 5 - 10 mice in each group. Data were analysed with One –way ANOVA, Tukey multiple comparisons test. BLM, Bleomycin; P, Progesterone; ns, non -significant, *** = P < 0.001, ** = P < 0.01, * = P < 0.05.