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. 2021 Nov 29;12:788317. doi: 10.3389/fmicb.2021.788317

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Copyright © 2021 Chen, Zhao, Wu, Wang, Wang, Pan, Hu, Pan and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of the lnc-LTR5B. (A) Schematic map of lnc-LTR5B. lnc-LTR5B is located on chromosome 17 and is flanked by the coding genes TUBB4B and ENTPD2L. (B) Dual-luciferase assays of lnc-LTR5B promoter activity in HEK293T cells transfected with the indicated plasmids. Schematic illustration of truncation constructs of lnc-LTR5B promoter region (−2000 to +198 relative to TSS). Data are presented as the mean±SD, n=3; ^**p<0.01, ^***p<0.001 (two-tailed unpaired Student’s t test). (C) Confocal RNA FISH images showing the distribution of lnc-LTR5B in DF-1 cells. The lnc-LTR5B probes labeled with CY3 (red), and nuclei are stained with 4', 6-diamidino-2-phenylindole (DAPI; blue), Scale bars, 10μm.