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. 2021 Nov 29;9:784367. doi: 10.3389/fcell.2021.784367

FIGURE 8.

FIGURE 8

Mitochondrial subunits of ERMES: Porin Mdm10 and Rho1 GTPase Gem1. (A) Mdm10 serves dual functions as component of ERMES and SAM. Transmembrane 19-stranded β barrel structure of Mdm10. The 19 β-strands are colored using a rainbow pattern and strands 1 and 19 forming the lateral gate of the barrel are labeled. Unresolved loops in the cryo-EM structure are indicated; L18 is the only large cytoplasmic loop present in Mdm10. Two views of the Mdm10 barrel colored in rainbow pattern except for the two opposite binding interfaces for ERMES (Y296-F298 and Y301) and SAM (Y73 and Y75) colored in magenta. (B) Domain organization of the tail-anchored mitochondrial GTPase Gem1 and Phyre 2 composite homology model based on the Drosophila/Homo Miro partial X-ray structures. Domains EF1, EF2 and C-GTPase2 domains are modelled “en blo” based on the Drosophila/Homo Miro structures while the N-GTPase1 domain is modelled based on the Homo Miro structure, although its exact relative orientation within the full-length Miro structure is not precisely known. Ca2+ (magenta) and Mg2+ (cyan) ions are drawn as spheres (PDB 7BTY-6D71-6KTY).