FIG. 1.
Expression of Bcl-xL and Bim in Baf-3 cells undergoing apoptosis induced by various death stimuli. (A to C) Baf-3 cells were cultured in IL-3-free medium (top panel), treated with 50 Gy of ionizing radiation (IR) and cultured with IL-3 (middle panel), or cultured in medium containing IL-3 and etoposide (VP16) at 3 μg/ml (bottom panel) for the indicated times. The expression of Bcl-xL protein (A), Bim mRNA (B), or Bim protein (C) was detected by specific antibodies for each protein (A and C) or by an RNase protection assay with a cDNA probe designed to detect mouse BimEL and BimL mRNA (B). (D) Percent viability, determined by trypan blue dye exclusion of Baf-3 cells treated with the various cell death inducers at the time of sample collection.