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. 2001 Feb;21(3):854–864. doi: 10.1128/MCB.21.3.854-864.2001

FIG. 2.

FIG. 2

Induction of apoptosis by each Bim isoform. (A) Immunoblot analysis of Baf-3 cells engeneered to express BimEL (lanes 1 and 2), BimL (lanes 3 and 4), or BimS (lanes 5 and 6) in the absence (lanes 1, 3, and 5) or presence (lanes 2, 4, and 6) of zinc (100 μM). Samples were collected 4 h after the addition of Zn. (B) Percent viability of cells expressing BimEL, BimL, or BimS after the addition of Zn (100 μM) in the presence of IL-3 (5 ng/ml). (C) Baf-3 cells inducibly expressing BimEL, pMT(BimELns clone 10), were cultured in IL-3-containing medium (5 ng/ml) with various Zn concentrations for 4 h (lanes 1 to 4). For comparison of expression levels, cell extracts from parental Baf-3 cells cultured in the absence of IL-3 for the indicated times (lanes 5 to 7) were blotted on the same membrane. Bim proteins were detected by immunoblot analysis. (D) Percent viability of pMT(BimELns clone 10) cells cultured in IL-3-containing medium (5 ng/ml) with various concentration of Zn.