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. 2021 Nov 29;12:773386. doi: 10.3389/fmicb.2021.773386

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Copyright © 2021 Umrekar, Winterborn, Sivabalasarma, Brantl, Albers and Beeby.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ArlG forms a helical filament. (A) Representative examples of purified P. furiosus sArlG demonstrating (i) helical filaments corresponding to previous results. Micrographs also featured (ii) thinner filamentous structures and (iii) helical filaments that transitioned to thinner filaments, mid-filament. (B) Representative 2-D classes on helical filaments; the bottom row shows 2-D classes of helical-to-thinner transitions, confirming their relation. (C) Isosurface of the final 3-D reconstruction of sArlG helical filament reveals an open helix of repeating densities around a central density.