TABLE 1.
Collection of electron cryomicroscopy images of purified sArlG filaments.
Data collection | |
Electron microscope | Titan Krios G2 |
Electron detector | Falcon III DE |
Voltage (keV) | 300 |
Magnification | 96,000 × (nanoProbe) |
Pixel size (Å) | 0.85 |
Defocus range (μm) | –1.5 to –2.9 |
Defocus step (μm) | 0.3 |
Dose rate (e–Å–2s–1) | 0.86 |
Integration time (s) | 40 |
Number of raw movies | 4632 |
Number of frames per movie | 32 |