Table 2.
Determination of IC50 against Miltefosine using MTT and Alamar Blue cell viability assay.
| Sl. No. | Groups | Isolate code | Alamar blue (IC50 in mM) | MTT (IC50 in mM) |
|---|---|---|---|---|
| 1 | 1. SSG-R/Mil-R/ Paro-R/AmpB-R | BHU 575 | 8.8 ± 1.4 | 9.7 ± 0.5 |
| 2 | BHU 814 | 13.1 ± 0.8 | 13.7 ± 0.5 | |
| 3 | 2. SSG-R/Mil-R, Paro-R | BHU 770 | 8.4 ± 1.3 | 8.0 ± 0.4 |
| 4 | BHU 872 | 17.8 ± 0.8 | 17.2 ± 0.9 | |
| 5 | BHU 782 | 16.5 ± 1.4 | 16.7 ± 1.4 | |
| 6 | 3. SSG-R/Mil-R | BHU 744 | 12.6 ± 0.9 | 12.5 ± 1.03 |
| 7 | 4. SSG-R/Paro-R | BHU 796 | 7.2 ± 1.2 | 6.9 ± 1.9 |
| 8 | 5. SSG-R/ AmpB-R | BHU 569 | 5.7 ± 0.6 | 4.1 ± 0.9 |
| 9 | 6. SSG-R | BHU 568 | 3.7 ± 0.3 | 3.7 ± 0.2 |
| 10 | BHU 764 | 3.5 ± 0.4 | 2.4 ± 0.3 | |
| 11 | BHU 572 | 3.3 ± 0.3 | 2.7 ± 0.2 | |
| 12 | BHU 573 | 7.1 ± 1.4 | 6.5 ± 1.7 | |
| 13 | BHU 574 | 12.4 ± 0.7 | 11.6 ± 0.6 | |
| 14 | 7. SSG-S/Mil-S, Paro-S/AmpB-S | BHU 816 | 2.9 ± 0.2 | 2.8 ± 0.6 |
| 15 | BHU 777 | 3.1 ± 0.8 | 3.1 ± 0.9 | |
| 16 | BHU 581 | 5.9 ± 0.8 | 4.5 ± 0.3 | |
| 17 | BHU 741 | 5.5 ± 0.3 | 6.1 ± 0.5 | |
| 18 | Ag 83 | 4.5± 0.8 | 5.1± 0.4 | |
| 19 | BPK 206 | 7.3 ± 0.7 | 6.5 ± 0.5 |
Low susceptibility towards SSG, Mil, Paro, and AmpB.
Low susceptibility towards SSG, Mil, and Paro.
Low susceptibility towards SSG and Mil.
Low susceptibility towards SSG and Paro.
Low susceptibility towards SSG and AmpB.
Low susceptibility towards SSG.
High susceptibility towards SSG, Mil, Paro, and AmpB.