Table 1.
Sequences of primers used for cloning and expression of flagellins and TLR5.
| Primer name | Sequence (5’−3’) | |
|---|---|---|
| FlaA | FlaA-F | ACAGCAAATGGGTCGCGGATCCACCATTACAGTAAATACTAACGTC (BamHI) |
| FlaA-R | GCAAGCTTGTCGACGGAGCTCTTACTGCAATAGTGACATTGCAG (SacI) | |
| FlaB | FlaB-F | ACAGCAAATGGGTCGCGGATCCGCAATTAATGTAAGCACTAACGTG (BamHI) |
| FlaB-R | GCAAGCTTGTCGACGGAGCTCTTAACCCAATAGACTAAGAGCTG (SacI) | |
| FlaC | FlaC-F | ACAGCAAATGGGTCGCGGATCCGCGGTTAATGTAAACACTAACGTT (BamHI) |
| FlaC-R | GCAAGCTTGTCGACGGAGCTCTTAACCAAGCAAACCAAGAGCAG (SacI) | |
| FlaD | FlaC-F | ACAGCAAATGGGTCGCGGATCCGCAGTTAATGTAAATACCAACGTA (BamHI) |
| FlaC-R | GCAAGCTTGTCGACGGAGCTCTTAGCCAAGTAAGCTAAGTGCCG (SacI) | |
| FlaE | FlaC-F | ACAGCAAATGGGTCGCGGATCCGCAATTACCGTTAATACCAATGTT (BamHI) |
| FlaC-R | GCAAGCTTGTCGACGGAGCTCCTAGCGAAGTAAGGCCAATGCTA (SacI) | |
| TLR5 | TLR5-F | ACAGCAAATGGGTCGCGGATCCACGTCGCTGGTTCTTCACTTG (BamHI) |
| TLR5-R | GCAAGCTTGTCGACGGAGCTCTTAGTTTTCTTCAAGTTTGAGAAAGTG (SacI) |
The meaning of the gray shadings is the restriction sites, and the restriction sites are in the brackets.