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Sequences of primers used for yeast two-hybrid analysis.
| Primer name | Sequence (5’−3’) | |
|---|---|---|
| FlaB | FlaB-F | GCCATGGAGGCCAGTGAATTCATGGCCATTAACGTTT (EcoRI) |
| FlaB-R | TGCAGCTCGAGCTCGATGGATCCACCCAACAAAGACAAA (BamHI) | |
| erTLR5 | erTLR5-F | GCATATGGCCATGGAGGCCGAATTCATGACGTCGCTGGTTCTTCACTTG (EcoRI) |
| erTLR5-R | GCGGCCGCTGCAGGTCGACGGATCCTCAGTTTTCTTCAAGTTTGAGAAAGTG (BamHI) |
The meaning of the gray shadings is the restriction sites, and the restriction sites are in the brackets.
