Table 3.

Sequences of primers used for site-directed mutagenesis of flaB.

FlaB	Abbreviation	Amino acid mutation	Sequence (mutation sites are shaded grey)
Q57	F1	Gln—Ala	F: 5’ CGTCAGCAAGCCGTGGCTTAG 3’
			R: 5’ GCCACGGCTTGCTGACGTTAAAC 3’
N83	F2	Asn—Ala	F: 5’ GGTGCAATGGCTGAAACCACCAA 3’
			R: 5’ GATATTGGTGGTTTCAGCCATTG 3’
N87	F3	Asn—Ala	F: 5’ CCACCGCTATCTTGCAACGTATG 3’
			R: 5’ CGTTGCAAGATAGCGGTGGTTTC 3’
R91	F4	Arg—Ala	F: 5’ CTTGCAAGCTATGCGAGATCTTTC 3’
			R: 5’ GATCTCGCATAGCTTGCAAGATA 3’
D94	F5	Asp—Ala	F: 5’ CGTATGCGAGCTCTTTCTTTG 3’
			R: 5’ GCAAAGAAAGAGCTCGCATA 3’
E122	F6	Glu—Ala	F: 5’ GATGCATTAAACCGTATTGCTGAA 3’
			R: 5’ GCAATACGGTTTAATGCATCATTG 3’
D152	F7	Asp—Ala	F: 5’ GGTGCCGCCTCTGGTGAA 3’
			R: 5’ CTTCACCAGAGGCGGCACCAAT 3’
N312	F8	Asn—Ala	F: 5’ CCAGGCCCGTTTCGGTCATG 3’
			R: 5’ GACCGAAACGGGCCTGGAATG 3’
R313	F9	Arg—Ala	F: 5’ CCAGAACGCTTTCGGTCAT 3’
			R: 5’ GACCGAAAGCGTTCTGGAATG 3’
N320	F11	Asn—Ala	F: 5’ GCCATCAGTGCCCTTGATAAC 3’
			R: 5’ CAAGGGCACTGATGGCATGA 3’
L97	F12	Leu—Asn	F: 5’ GAGATCTTTCTAACCAATCAGCA 3’
			R: 5’ CGTTTGCTGATTGGTTAGAAAG 3’
H316	F13	His—Ala	F: 5’ CGTTTCGGTGCTGCCATCAGTA 3’
			R: 5’ GGTTACTGATGGCAGCACCGAAA 3’
I324	F14	Ile—Asn	F: 5’ GATAACAACAACGAGAACGTCAA 3’
			R: 5’ CGTTCTCGTTGTTGTTATCAAGGT 3’