Table 4.

Calculated dissociation constant (Kd) values flaB mutants binding to TLR5 protein inferred by SPR.

FLAB	Ligand/receptor	Dissociation constant(Kd, μM)
WT	WT/TLR5	3.283
F1	F1/TLR5	0.4314
F2	F2/TLR5	0.4472
F3	F3/TLR5	26.23
F4	F4/TLR5	2.571
F5	F5/TLR5	46.34
F6	F6TLR5	4.403
F7	F7/TLR5	71.68
F8	F8/TLR5	3.796
F9	F9/TLR5	14.76
F11	F11/TLR5	12.96
F12	F12/TLR5	2.411
F13	F13/TLR5	7.29
F14	F14/TLR5	27.48