FIGURE 1.

SNS inhibits CUMS-mediated lung metastasis of breast cancer in 4T1 tumor-bearing mice. (A) Flowchart of the experiment (SNS^Low, 0.825 g/kg; SNS^High,1.65 g/kg). (B) The representative movement trajectory of the mice (n = 8, upper panel), as well as the total distance, and percentage of sucrose water consumed (lower panel). (C) Tumor growth curves (upper panel) and body weight changes (lower panel) of the 4T1 tumor-bearing mice with different treatments (n = 8). (D) In vivo imaging of lung metastatic lesions in the 4T1 tumor-bearing mice with different treatments (left panel). The intensity of bioluminescence was quantified by an IVIS-spectrum system (n = 8, right panel). (E) Representative pictures of gross lung metastatic lesions in the 4T1 tumor-bearing mice with different treatments. The number of metastatic lesions was quantified (n = 8, upper panel). HE staining of the lung metastatic lesions in the 4T1 tumor-bearing mice with different treatments. The black circle indicates the metastatic lesions of the lung. The scale bars indicate 1000 μm. The metastatic lesion area was quantified by ImageJ software (n = 8, lower panel). Data are represented as the mean value ±SD. One-way ANOVA and Bonferroni’s post hoc test were applied. ANOVA for repeated measurements was performed in (C).