Figure 1.

Coding RNA sample clustering and tissue-specific enrichment in control animals. Principal component analysis (PCA) 2-dimensional (2D) and 3-dimensional (3D) score plots and orthogonal partial least square discriminant analysis (OPLS-DA) score plots for the liver and muscle from control animals are shown at the top. For the PCA the 2D and 3D plots are plotted with principal component 1 on the x axis and principal component 2 on the y axis, and for the OPLS-DA score plot with predictive component on the x axis and first orthogonal component on the y axis, showing separation between the liver (brown) and muscle (pink) from control animals. Each point represents one animal. The volcano plot showing differential gene expression comparing liver and muscle in control animals are shown at the bottom left. Genes are plotted by log2 fold change and –log10 adjusted P values. The orange points represent genes that have an absolute log2 fold change greater than 0.5 and false discovery rate (FDR) less than 0.05. Heat map of differentially regulated gene pathways across both liver and muscle at FDR less than 0.01 are shown at the bottom right.