Fig 2. GC inoculation induces distinct F-actin reorganization in non-polarized and polarized epithelial cells.
Non-polarized and polarized T84 cells were apically inoculated with Pil+Opa+, OpaCEA or ΔOpa GC (MOI~10) from the top chamber for 6 h. Cells were fixed, stained for F-actin and GC, and analyzed using 3D-CFM. (A) Representative xy images of the top surface and xz images crossing the top and the bottom surfaces are shown. Arrows indicate the location of GC. (B) The redistribution of F-actin was quantified by the mean fluorescence intensity ratio (FIR) (±SEM) of F-actin underneath individual GC microcolonies relative to the adjacent no GC surface area. Data points represent individual GC microcolonies. Scale bar, 20 μm. n = 3 three independent experiments. *p<0.05; **p< 0.01; ***p<0.001.