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. 2021 Dec 1;17(12):e1009592. doi: 10.1371/journal.ppat.1009592

Fig 5. GC differentially remodel the surface morphology of non-polarized and polarized epithelial cells in an ezrin- or NMII-dependent manner.

Fig 5

Non-polarized and polarized T84 cells were pre-treated with or without NSC668394 (20 μM) or ML-7 (10 μM) and incubated with Pil+Opa+ or Pil+ΔOpa GC (MOI~50) in the apical chamber for 6 h with or without the inhibitors. Cells were fixed and processed for TEM. (A, C) Shown are representative images with high (left panels) and low (right panels) magnifications. Yellow dash line rectangles highlight the focused area. Solid arrows, GC-associated elongated microvilli. Open arrows, GC contacting bending microvilli. Open arrowheads, no microvilli visible in GC contact sites. (B, D) Membrane morphological changes were quantified by the percentage of GC associating with elongated microvilli in non-polarized epithelial cells (B) and the percentage of GC associating with the plasma membrane of polarized epithelial cells that lost microvilli (D). Data points represent individual randomly acquired TEM images. Scale bar, 1 μM. n = 2–3 two to three independent experiments. *p<0.05; ***p<0.001.