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. 2021 Dec 1;17(12):e1009592. doi: 10.1371/journal.ppat.1009592

Fig 8. GC inoculation induces ezrin and NMII redistribution in the polarized endocervical epithelial cells, leading to F-actin reduction at GC adherent sites.

Fig 8

Human cervical tissue explants were pretreated with or without ML-7 and incubated with Pil+Opa+ and Pil+ΔOpa GC (MOI~10) for 24 h in the absence or presence of ML-7 (10 μM). Tissue explants were stained for F-actin by phalloidin, ezrin, pMLC, GC and DNA, and analyzed using 3D-CFM. (A, D) Shown are representative images of ezrin (A) and F-actin (D) staining that intercept both the apical and basolateral surfaces of endocervical epithelila cells. Scale bar, 20 μm. (B, C) The redistribution of ezrin in the endocervical epithelium was quantified by the FIR (±SEM) of ezrin staining underneath individual GC microcolonies relative to the adjacent no GC surface area (B) and by the FIR at the apical surface relatively to the cytoplasm (C). (E) The redistribution of F-actin was quantified by the FIR (±SEM) of phalloidin staining underneath individual GC microcolonies relative to the adjacent no GC area. Data points represent individual GC microcolonies. n = 3 three cervixes. **p< 0.01; ***p<0.001.