Table 4.

Least squares means for the effect of lubabegron (LUB) on cumulative NH₃ gas emissions and standardized cumulative NH₃ gas emissions corresponding to each BW measurement¹

	LUB (mg·kg−1 DM)						Dose	Significance of contrast
Variable	0.0	1.38	5.5	22.0	SEM	Dose × sex P-value2	P-value2	Control vs. 1.38 mg·kg−1	Control vs. 5.5 mg·kg−1	Control vs. 22.0 mg·kg−1
Unshrunk BW, kg
Initial	451	454	455	452	10.0	0.996	0.937
Day 7	464	469	470	469	10.4	0.968	0.935
Day 14	474	484	484	478	12.3	0.962	0.655
Day 28	496	508	506	501	12.2	0.990	0.627
Day 56	529	545	542	539	15.2	0.974	0.411
Final	567	583	582	582	19.4	0.916	0.257
NH3, g
Days 0 to 7	415	394	387	326	49	0.746	0.022	0.439	0.300	0.004
Days 0 to 14	953	835	801	699	106	0.802	0.006	0.062	0.020	< 0.001
Days 0 to 28	2,097	1,783	1,686	1,563	265	0.573	0.006	0.027	0.006	< 0.001
Days 0 to 56	4,619	4,089	3,888	3,763	540	0.420	0.019	0.050	0.011	0.004
Days 0 to 91	7,783	7,093	6,860	6,751	855	0.281	0.052	0.076	0.023	0.013
NH3, g/kg BW
Days 0 to 7	0.89	0.84	0.82	0.70	0.106	0.619	0.003	0.278	0.139	<0.001
Days 0 to 14	2.01	1.73	1.66	1.47	0.223	0.698	<0.001	0.010	0.002	<0.001
Days 0 to 28	4.21	3.53	3.33	3.13	0.509	0.463	<0.001	0.004	<0.001	<0.001
Days 0 to 56	8.66	7.50	7.14	6.99	0.871	0.258	<0.001	0.004	<0.001	<0.001
Days 0 to 91	13.6	12.2	11.7	11.6	1.19	0.161	0.004	0.009	0.002	<0.001

¹Emissions were measured from an individual CPE (n = 8) over 15-min sampling periods using calibrated, gas-specific analyzers (Thermo Environmental Instruments (TEI), Waltham, MA). This procedure was continuous so that a maximum of 11 sampling periods were available for determination of daily emission rates/animal for each CPE. Daily gas emissions were defined as those within the span beginning at 0800 hours and ending at 0759 hours the next morning. Cumulative emissions were standardized by the BW measured on the day corresponding to the emissions time-period for reporting.

²If the dose × sex interaction was not significant (P > 0.05), contrasts were constructed to evaluate the main effect of dose for the pooled sexes. Statistical significance for dose was declared when P ≤ 0.05 and tendencies were declared when 0.05 < P ≤ 0.10.