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. 2021 Dec 2;10:e71656. doi: 10.7554/eLife.71656

Figure 2. Cross-linking mass spectrometry analysis of the inter-subunit interactions within the five-subunit tRNA-LC (RTCB:DDX1:FAM98B:CGI-99:ASW).

(A) Inter-subunit cross-links between RTCB, DDX1, CGI-99, and FAM98B subunits within the five-subunit full tRNA-LC are shown as dashed lines. (B) Inter-subunit cross-links between ASW and the remaining subunits of the full tRNA-LC. Domain schematic as in Figure 1A. The dashed lines indicate cross-links observed at least once in two replicates.

Figure 2—source data 1. Cross-links identified in the cross-linking and mass spectrometric (XL-MS) analysis of the full tRNA-LC.
Figure 2—source data 2. Cross-links identified in the cross-linking and mass spectrometric (XL-MS) analysis of the core tRNA-LC.

Figure 2.

Figure 2—figure supplement 1. Cross-linking mass spectrometry analysis of tRNA-LC.

Figure 2—figure supplement 1.

(A) Intra-molecular cross-links identified in the five-subunit tRNA-LC sample are shown as dashed lines. (B) Inter-molecular cross-links identified in the four-subunit core tRNA-LC containing RTCB, DDX1, FAM98B, and CGI-99 subunits. Domain composition is indicated as in Figure 1A. The dashed lines indicate cross-links observed at least once in two replicates.