Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | Tg(Vil1-cre/ ERT2)23Syr |
el Marjou et al., 2004 | RRID:MGI:3053826 | |
| Genetic reagent (Mus musculus) | Apctm1Tno | Shibata et al., 1997 | RRID:MGI:1857966 | |
| Genetic reagent (Mus musculus) | Krastm4Tyj | Jackson et al., 2001 | RRID:MGI:2429948 | |
| Genetic reagent (Mus musculus) | Rpl24Bst | Oliver et al., 2004 | RRID:MGI:1856685 | |
| Genetic reagent (Mus musculus) | Eef2kD273A | Gildish et al., 2012 | ||
| Genetic reagent (Mus musculus) | ApcMin | Moser et al., 1990 | RRID:MGI:1856318 | |
| Cell line (Mus musculus) | VillinCreER
Apcfl/fl
KrasG12D/+ small intestinal organoids |
This study | ||
| Cell line (Mus musculus) | VillinCreER
Apcfl/fl
small intestinal organoids |
This study | ||
| Cell line (Mus musculus) | VillinCreER
Apcfl/fl
KrasG12D/+ Rpl24Bst/+ small intestinal organoids |
This study | ||
| Cell line (Mus musculus) | VillinCreER
Apcfl/fl
Rpl24Bst/+ small intestinal organoids |
This study | ||
| Antibody | BrdU(mouse monoclonal) |
BD Biosciences #347,580 |
RRID:AB_400326 | IHC: (1:250) |
| Antibody | P-eEF2 T56(rabbit polyclonal) |
Cell Signaling Technology #2,331 |
RRID:AB_10015204 | WB: (1:2000)IHC: (1:100) |
| Antibody | eEF2(rabbit polyclonal) |
Cell Signaling Technology #2,332 |
RRID:AB_10693546 | WB: (1:2000) |
| Antibody | P-4E-BP1(rabbit monoclonal) |
Cell Signaling Technology #2,855 |
RRID:AB_560835 | IHC: (1:250) |
| Antibody | P-RPS6 S240/4(rabbit monoclonal) |
Cell Signaling Technology #5,364 |
RRID:AB_10694233 | IHC: (1:100) |
| Antibody | P-eIF2α S51(rabbit monoclonal) |
Cell Signaling Technology#3,398 |
RRID:AB_2096481 | IHC: (1:50) |
| Antibody | P-ERK T202/ Y204(rabbit polyclonal) |
Cell Signaling Technology #9,101 |
RRID:AB_331646 | IHC: (1:400) |
| Antibody | Lysozyme(rabbit polyclonal) | Dako A0099 | RRID:AB_2341230 | IHC: (1:300) |
| Antibody | RPS6(mouse monoclonal) |
Cell Signaling Technology #2,317 |
RRID:AB_2238583 | WB: (1:2000) |
| Antibody | RPL10(rabbit polyclonal) |
Novus NBP1-84037 |
RRID:AB_11007661 | WB: (1:2000) |
| Antibody | RPL22(rabbit polyclonal) |
Abcam ab111073 |
RRID:AB_10863642 | WB: (1:2000) |
| Antibody | Acetyl-CoA carboxylase(rabbit monoclonal) |
Cell Signaling Technology #3,676 |
RRID:AB_2219397 | WB: (1:1000) |
| Antibody | P-acetyl-CoA carboxylase S79(rabbit monoclonal) |
Cell Signaling Technology #3,661 |
RRID:AB_330337 | WB: (1:1000) |
| Antibody | β-Actin(mouse monoclonal) |
Sigma-Aldrich #A2228 |
RRID:AB_476697 | WB: (1:10,000) |
| Antibody | β-Tubulin(mouse monoclonal) |
Cell Signaling Technology #2,128 |
RRID:AB_823664 | WB: (1:4000) |
| Antibody | Goat Anti-Mouse Immunoglobulins/ HRP(goat polyclonal) |
Dako #P0447 | RRID:AB_2617137 | WB: (1:2000) |
| Antibody | Goat Anti-Rabbit Immunoglobulins/ HRP(goat polyclonal) |
Dako #P0448 | RRID:AB_2617138 | WB: (1:2000) |
| Sequence- based reagent |
Olfm4 RNAScope | ACD #311,838 | RNAScope | |
| Peptide, recombinant protein |
Recombinant Murine Noggin |
Peprotech #250-38 | 100 ng/ml | |
| Peptide, recombinant protein |
Animal-Free Recombinant Human EGF |
Peprotech #AF-100-15 |
50 ng/ml | |
| Peptide, recombinant protein |
Recombinant Mouse R-Spondin 1 Protein |
R&D Systems #3474-RS |
500 ng/ml | |
| Commercial assay or kit |
Vectorstain Elite ABC-HRP |
Vector Laboratories PK-6102 | RRIDs:AB_2336820 | |
| Commercial assay or kit |
Vectorstain Elite ABC-HRP |
Vector Laboratories PK-PK-6101 |
RRIDs:AB_2336821 | |
| Commercial assay or kit |
Cell Proliferation Labelling Reagent |
Amersham Bioscience RPN201 |
||
| Commercial assay or kit |
Cell-Titer Blue | Promega #G8080 | ||
| Chemical compound, drug |
Rapamycin | LC Laboratories #R-5000 |
||
| Chemical compound, drug |
Harringtonine | Santa Cruz sc-204771 |
||
| Chemical compound, drug |
EasyTag EXPRESS 35S Protein Labeling Mix |
Perkin Elmer #NEG772002MC | ||
| software, algorithm |
Image J | Rueden et al., 2017 | RRID:SCR_003070 | |
| software, algorithm |
G*Power | Faul et al., 2009 | RRID:SCR_013726 |