Figure 3.

Non-Negative Least Squares (NNLS) Haplotype method

The NNLS Haplotype method exhibits a bar plot of the ancestral composition for a set of surrogate groups, as demonstrated in the legend. Implemented in ChromoPainter (CP) and merged with HGDP sequences, each individual (recipient) was painted as a combination of genomic fragments inherited by all the HGDP panel (donors). Thirty-two Hispanic RDEB patient samples carrying common mutations in the COL7A1 gene sorted according to Middle Eastern ancestry from the Southwestern United States, Mexico, Chile, and Colombia (Figure 3a). Asterisks in Figure 3a highlight the samples shown in Figure 3b. The analysis of RDEB samples with affinity to Sephardic origins sorted according to Middle Eastern ancestry demonstrate North African and Middle Eastern origins, highlighted in green, effectively supporting hidden genetic ancestry in heavily admixed individuals (Figure 3b).