Figure 4.

Ycs4 interface with Brn1-Ycg1. (A) Identically labeled (green) or unlabeled (light green) lysines of Ycs4 alone and in the Ycs4-BY complex. (B) Differentially labeled Ycs4 lysines in the protein alone and in the complex with Brn1 and Ycg1 (Ycs4-BY). Light blue-colored lysines remained undetected in the Ycs4-BY complex but were labeled in Ycs4 alone. Lysines colored in dark blue are labeled in Ycs4 but remain unlabeled in the Ycs4-BY complex. Teal lysines were accessible in Ycs4 but not in the Ycs4-BY complex, while orange-colored lysines became exposed in the complex. (C) Surface mapping of lysines labeled identically (green) or differentially (blue, purple, teal, and orange) along with the previously¹ described lysines involved in the Ycs4-Brn1 interaction (yellow).