Figure 4.

Effects of sRAGE on the transcription of IL-1β mRNA and IL-18 mRNA in primary cultured cardiomyocytes following ischemia-reperfusion injury. I/R groups were subjected to ischemia for 2 hours and reperfusion for 2 hours. (a) Quantitative RT-PCR of interleukin-1β mRNA expression in cardiomyocytes. (b) Quantitative RT-PCR of interleukin-18 mRNA expression in cardiomyocytes. β-Actin was used as the reference gene. Data are expressed as mean ± SEM (n = 3 replicates). ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ns: no significance.