FIG. 2.

MMS sensitivity of the rad51Δ strain transformed with the different rad51 mutants. Two mutants (G210C and A248T) showed the highest sensitivity to MMS, indistinguishable from that of the rad51Δ strain. The sensitivity of all other mutants (not shown, for clarity) fall in the area indicated by the arrow and demarcated by mutants Y388H and L99P. Data in the figure are displayed as the fraction of colonies viable in the presence of MMS relative to the non-MMS control plates. At least three independent experiments were carried out, and the values obtained from each experiment were averaged. Control, pRS413; scRAD51, pRS413-RAD51.