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. 2021 Dec 13;11:23907. doi: 10.1038/s41598-021-03404-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TBK1 phosphorylation of p62 at S403 promotes its aggregates formation in FIP200 KO cells specifically. (A) Representative immunofluorescence images for phosphorylated TBK1 and p62 (S403) in WT and FIP200 KO HeLa cells. (B,C) Quantification of (B) pTBK1 + fluorescence intensity per cell and (C) pp62(403) + and pTBK1 + puncta number per WT and FIP200 KO cell. (D) Representative immunofluorescence images for mCherry fused p62 and p62 (S403A) mutant as well as their phosphorylated versions (pp62 and pp62(S403A) mutant) in WT and FIP200 KO HeLa cells, respectively. (E) Quantification of p62 and p62 (S403A) puncta size per WT and FIP200 KO cell. (F) Representative immunofluorescence images of p62 in WT and FIP200 KO HeLa cells expressing empty Vector, FIP200 or FIP200-4A. (G) Quantification of p62 + puncta size per cell in (F). Scale bars: 10 µm. *, P < 0.05; **, P < 0.01; ****, P < 0.0001. Graphs represent mean ± SEM.