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. 2021 Dec 13;4:1390. doi: 10.1038/s42003-021-02919-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Only tumour cells with a complete nucleus are considered here. Blood capillary portions are in red. a Accumulation map of virtual rays [Accumulated ray intensity [a.u]; voxel-by-voxel basis; see yellowish voxels] emitted by each cell along its main axis. (i) Reconstructed 3D image of accumulation map of virtual rays. (ii–ix) 2D cross-sectional maps for increasing depth along the Z-axis. b Binary classification of polarised/unpolarised cells: (i) Reconstructed 3D image of polarised/unpolarised cells. 17 cells (in yellow) emitted virtual rays reaching the main accumulation region observable in panel (vii) (accumulated ray intensity >3.5). a.u., arbitrary unit. (ii–ix) 2D cross-sectional maps for increasing depth along the Z-axis. Scale bar = 10 µm.