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. 2021 Dec 13;12(12):1153. doi: 10.1038/s41419-021-04437-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A THP-1 cells were labeled with a lncTRPM2-AS probe (red) and negative control (NC) probe (red) using fluorescence in site hybridization (FISH), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (nucleus staining, blue). B Confirmation of decreased lncTRPM2-AS levels following lncTRPM2-AS shRNA treatment. C Detection of cell proliferation in shNC or shlncTRPM2-AS (shAS) cells with the CCK-8 assay. D Detection of the apoptosis in shNC and shAS cells with the Annexin V/7-AAD assay. E Detection of the levels of IL-1β, IL-4, IL-6, IL-10, TNF-α, and TGF-β in shNC and shAS cells by RT-PCR. Bars, ± SD; n = 3, *P < 0.05; **P < 0.01; ***P < 0.001.