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. 2021 Dec 13;12(12):1153. doi: 10.1038/s41419-021-04437-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Potential ubiquitination sites of TRPM2 obtained from http://plmd.biocuckoo.org/. B, C Western blot analysis of TRPM2 in HEK293T cells co-transfected with Flag-Trim21, HA-Ub, and wild type or mutant TRPM2 (TRPM2 mutants: K8R, K55R, K117R, K307R, K314R, K405R, K423R, K596R, K703R, K1218R, and K1544R). D Immunoprecipitation analysis of ubiquitination of TRPM2 in HEK293T cells co-transfected with HA-Ub, Flag-TRIM21, and TRPM2 (WT or K1218R). E RIP experiments were used to detect the amount of lncTRPM2-AS after IP TRPM2. F RIP experiments were used to detect the amount of lncTRPM2-AS after IP Myc which represent different domains of TRPM2 as indicated. Bars, ± SD; n = 3, ns not significant; ***P < 0.001.