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. 2021 Dec 13;7:114. doi: 10.1038/s41531-021-00259-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Representative electron micrograph showing the synaptic ultrastructure (white arrows) and mitochondria (blue arrows) in neurons. Scale bars = 500 nm. b Quantitative analysis of the area of the presynaptic membrane in the two groups of mice (control: n = 51 synapses from 4 mice; hα-syn: n = 49 synapses from 4 mice). c Quantitative analysis of the mitochondrial aspect ratio (control: n = 231 mitochondria from 4 mice; hα-syn: n = 343 mitochondria from 4 mice). d Representative Golgi staining photomicrograph showing neurons in the granule cell layer of the OB. Scale bars = 20 μm. e Representative reconstructions of granule cells from the OB of mice in the two groups. f Comparison of the total dendritic length of granule cells in the two groups of mice (control: n = 29 cells from 4 mice; hα-syn: n = 32 cells from 4 mice). g Representative dendrites and spines of granule cells obtained from control and hα-syn mice. Scale bars = 2 μm. h Spine density on granule-cell dendrites (control: n = 39 dendrites from 3 mice; hα-syn: n = 42 dendrites from 3 mice). **p < 0.01; ***p < 0.001; n.s. not significant. Data are presented as mean ± SEM.