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. 2021 Dec 13;6:151. doi: 10.1038/s41541-021-00414-4

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Schematic representation of the SARS-CoV-2 spike protein showing the different domains with amino acid (aa) numbers. Red asterisk denotes the identified H2K^b/D^b-restricted epitopes. b Amino acid residues of SARS-CoV-2 glycoprotein peptide pools. Matrix pools (4, 5, and 38) and overlapping peptide pools of 15-mer peptides overlapping by 11 aa (19, 24, and 48) containing 315 peptides across the entire glycoprotein were tested in the ELISpot assay. Reactivity to specific matching sequences in pools are highlighted with different matching colors and represent the predicted epitopes for H2K^b/D^b (iedb.org) or predicted epitopes without ELISpot responses (blue highlight; hypothetical epitope). Amino acid sequences 13–27 (pool 4) and 17–31 (pool 5) have been highlighted in color to indicate confirmed epitopes based on ELISpot reactivity to peptide pool 14. c Number of spike-specific IFN-γ spot forming units stimulated with 52 peptide pools from the Epitope Mapping Peptide Set of the spike protein is presented as scatter plots (mean ± s.d./4 × 10⁵ splenocytes). ELISpot images of triplicate wells and the stimulants are shown on the X-axis. T cell responses were considered positive when mean spot count exceeded the mean ± 3 s.d. of the negative control wells. Splenocytes were stimulated in vitro with media (negative control), anti-CD3 (positive control), or SARS-CoV-2 spike peptide pools 4, 5, 19, 24, 38, and 44.