Fig. 9. Flow cytometry-based CFSE cytotoxic T cell (CTL) assay to measure antigen-specific killing in response to SpFN vaccine at week 6 following prime-boost vaccination.

Flow cytometry plots depicting the frequency of a CFSE-high/CFSE-low population and b effector cells from the spleens of mice vaccinated with SpFN vaccine adjuvanted with ALFQ or AH. c Graph depicting the ratio of CFSE high to low (indicating antigen-specific killing) as a function of effector cell dilution. Each curve represents effector cell dilutions from a single mouse vaccinated with SpFN + ALFQ or SpFN + AH. The curves were evaluated using the Pearson correlation coefficient. Two independent experiments were performed and data from one of the experiments is shown.