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. 2021 Dec 13;11:23852. doi: 10.1038/s41598-021-03288-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Screening results for cytochromes bd-I (light-grey), bd-II (dark-grey) and bo₃ (white) for short-chain AurD-type compounds. (B) Screening results for selected AurD-type compounds with decreasing residual activity for cytochrome bo₃ from left to right. Inhibition assay using test compounds at 250 µM in presence of 200 µM ubiquinone-1 and 5 mM dithiothreitol. Oxygen reduction activity was calculated from oxygen consumption rates at RT. Reference activity (100%) of each oxidase was determined in presence of DMSO to exclude secondary effects of the solvent. Presented data are mean ± S.E.M. (n = 3) ***p < 0.001, **p < 0.01, *p < 0.05, ^NSp > 0.05.