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. 2021 Dec 13;4:1391. doi: 10.1038/s42003-021-02906-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a PC9, H1299, A549, HCC827, and NCI-H1975 cells were treated with a range of concentrations of D6 for 72 h. Cell viability was detected by CCK8 and shown as relative viability compared to the control. b, c Representative images showed the cell proliferation of NCI-H1975, A549, and PC9 cells exposed to D6 (2.5 µM) and solvent DMSO. The bars of (c) represented the relative cell density. d HCC827 parental cells or the isogenic cells expressing L858R/T790M-EGFR were treated with the indicated concentrations of D6 for 72 h. Cell viability (%) was shown after normalized to the control group. e Representative images showed the cell growth of NCI-H1975 cells cultured in soft agar with the indicated concentrations of D6. Scale bar, 200 µm. f Immunoblotting analysis showing the levels of cleaved-PARP (cl-PARP) in NCI-H1975 cells treated with indicated doses of D6. g Quantitative PCR analysis showing the levels of anti-apoptosis genes in NCI-H1975 cells in the presence of D6 or not. h Representative images showing the effect of D6 on NCI-H1975 cells and normal lung fibroblast MRC5 cells. Scale bar, 200 µm. Data are presented as mean ± SEM (a, c, and g), n = 3 (a and g) or 6 (c) biologically independent samples. P values were calculated by two-tailed Student’s t-test.