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. 2021 Dec 13;11:631. doi: 10.1038/s41398-021-01745-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Injection schematic and lentiviral constructs. CMV, human cytomegalovirus promoter; EGFP, enhanced green fluorescent protein; WPRE, Woodchuck hepatitis virus posttranscriptional regulatory element; Olig1, oligodendrocyte transcription factor 1. B Experimental timeline. BrdU, bromodeoxyuridine; LD, light–dark box. C Representative image of viral spread through the dorsal dentate gyrus. Lenti, lentivirus; DAPI, 4′,6-diamidino-2-phenylindole; GFP, green fluorescent protein. Scale bar = 200 μm. D Representative images of GFP and Olig1 immunohistochemistry 7 weeks post injection. Scale bar = 50 μm. E Dentate gyrus Olig1 cell densities (GFP: 28.11 ± 2.33 cells/mm², Olig1: 41.54 ± 3.72 cells/mm²; two-tailed t test: t(10) = 3.06, p = 0.012; n = 6 per group). F Composite behavior scores (GFP: 4.4 ± 0.9, Olig1: 9.7 ± 2.0; two-tailed t test: t(14) = 2.58, p = 0.022; n_GFP = 9, n_Olig1 = 7). G Composite behavior scores from avoidance tests (GFP: 3.3 ± 0.7, Olig1: 7.0 ± 1.6; two-tailed t test: t(14) = 2.21, p = 0.044; n_GFP = 9, n_Olig1 = 7). H Pearson correlation of Olig1 cell densities to avoidance composite scores from Lenti-Olig1 animals (Pearson correlation: r = 0.86, p = 0.028; n = 6). I Composite behavior scores from the acoustic startle response (ASR) test (GFP: 1.1 ± 0.5, Olig1: 2.7 ± 0.5; two-tailed t test: t_2,14 = 2.24, p = 0.042; n_GFP = 9, n_Olig1 = 7). J Pearson correlation of Olig1 cell densities to composite startle scores from Lenti-Olig1 animals (Pearson correlation: r = 0.23, p = 0.66; n = 6). For all plots, individual data points are shown with mean ± SEM, *p < 0.05.