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. 2021 Nov 19;297(6):101428. doi: 10.1016/j.jbc.2021.101428

Figure 5.

Figure 5

Effect of the TSC1/2 complex on the GTPase activity of WT and mutant RHEB proteins.A, representative chromatograms of the GTPase assay using IP-RP-HPLC. The GST-fusion proteins of WT RHEB were incubated at 30 °C without (left panel) or with (right panel) Flag-TSC1/2 immunoprecipitation beads at the indicated times, and the aliquots were subjected to IP-RP-HPLC analysis. B, the GST-fusion proteins of the WT and mutant forms of RHEB were incubated at 30 °C without (open circle) or with (closed circle) Flag-TSC1/2 immunoprecipitation beads at the indicated times, and the aliquots were subjected to IP-RP-HPLC analysis. The data are the means from three independent experiments. The error bars are not shown as the standard deviations of the data were within 1%. ∗ p < 0.05, Student’s t test. IP-RP-HPLC, ion-pair reversed-phase HPLC; RHEB, Ras homolog enriched in brain.