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. 2021 Dec 13;11:23806. doi: 10.1038/s41598-021-03325-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Deep sequencing analysis of the MSTN target region in delivered piglets. *Nucleotides in blue and red represent the target sequences and PAM sequences of gRNA, respectively. Nucleotides in green represent inserted nucleotids. **The read frequency was defined as the ratio of the number of reads to the total number of aligned read. ***The total mutation rate was defined as the ratio of the total number of modified reads to the total number of aligned reads. WT wild-type; ♂, male.