Table 1.
Frequencies of unmodified sequences at candidate off-target sites analyzed by deep sequencinga.
| Piglet | MSTN_OT1 (%) | MSTN_OT2 (%) | MSTN_OT3 (%) |
|---|---|---|---|
| Controlb | 22,986/23,492 (97.85) | 27,329/27,649 (98.84) | 22,216/22,512 (98.69) |
| #1 | 17,469/17,785 (98.22) | 18,918/19,165 (98.71) | 13,355/13,495 (98.96) |
| #2 | 20,386/20,833 (97.85) | 18,798/19,049 (98.68) | 16,906/17,143 (98.62) |
| #3 | 20,346/20,739 (98.11) | 19,799/20,038 (98.81) | 18,951/19,193 (98.74) |
| #4 | 22,710/23,187 (97.94) | 18,717/18,928 (98.89) | 19,321/19,592 (98.62) |
| #5 | 19,655/20,030 (98.13) | 21,168/21,429 (98.78) | 19,918/20,152 (98.84) |
| #6 | 17,406/17,702 (98.33) | 21,302/21,529 (98.95) | 12,042/12,167 (98.97) |
| #7 | 17,653/17,978 (98.19) | 20,003/20,250 (98.78) | 20,341/20,550 (98.98) |
| #8 | 19,605/20,006 (98.0) | 21,832/22,117 (98.71) | 11,236/11,351 (98.99) |
| #9 | 24,726/25,196 (98.13) | 25,132/25,430 (98.83) | 19,907/20,157 (98.76) |
aThe frequency was defined as the ratio of the number of unmodified reads to the total number of aligned reads.
bA DNA sample derived from a wild-type pig, not subjected to any gene editing technique, was used as the control.