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. 2021 Dec 13;12:7216. doi: 10.1038/s41467-021-27451-w

Fig. 3. Depletion of Nup210 in 4T1 metastatic cancer cells reduces lung metastasis in mice.

Fig. 3

a Western blot of Nup210 knockdown (KD) in 4T1 cells. b Primary tumor weight, c representative lung images, and d lung metastases count and lung metastases count normalized to primary tumor weight in orthotopically transplanted Nup210 KD 4T1 cells. ANOVA with Tukey’s multiple comparison test, mean ± s.d. sh-Ctrl, n = 10; sh # 1, n = 10; sh # 4, n = 9 mice. e Western blot showing CRISPR/Cas9-mediated knockout (KO) of Nup210 in 4T1 cells. f Primary tumor weight, g representative lung images, h lung metastases count and tumor-normalized metastases count after orthotopic transplantation of Nup210 KO 4T1 cells. ANOVA with Tukey’s multiple comparison test, mean ± s.d. n = 10 mice per group. i DNA content analysis with EdU incorporation of the different stages of the cell cycle in sg-Ctrl and Nup210 KO 4T1 cells. j Quantification of cell cycle stage distribution in sg-Ctrl and Nup210 KO 4T1 cells.Two-tailed t test, mean ± s.e.m, n = 3 biological replicates. k Western blot (left) and qRT-PCR (right) of NUP210 overexpression in 4T1 cells. Two-tailed t test, mean ± s.e.m, n = 3 biological replicates. l Primary tumor weight, m representative lung images, and n lung metastases count after orthotopic transplantation of NUP210-overexpressing 4T1 cells. Mann–Whitney U test, mean ± s.d. n = 10 mice per group.