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. 2021 Dec 13;12:7216. doi: 10.1038/s41467-021-27451-w

Fig. 7. NUP210 is mechanosensitive and regulates tumor cell migration and invasion.

Fig. 7

a Representative images of 4T1 cells on fibronectin-coated hydrogel layers of soft (0.2 kPa) and stiff (12 kPa) matrices. Scale bar = 10 μm. b (Left) Western blot of NUP210, p-FAK Y397, and T-FAK (Total FAK) proteins of 4T1 cells grown on soft or stiff matrices. (Right) Quantification of signals normalized to T-FAK intensity. c qRT-PCR of Nup210-regulated and known mechanosensitive genes (Ier3, Mrtf-a, and Yap1) genes in soft and stiff matrices. Multiple two-tailed t test, mean ± s.e.m. n = 3 biological replicates. d Morphology of sh-Ctrl and Nup210 KD 4T1 cells in soft and stiff matrices. Scale bar = 10 μm. e Live-cell imaging of F-tractin-MLC2 reporter transduced 6DT1 sh-Nup210 cells. Cells were treated with cytochalasin D for 1 h and imaged overnight after drug washout. Scale bar = 10 μm. f (Left) cell migration tracks (red) of sg-Ctrl and Nup210 KO 4T1 cells. (Right) Quantification of cell speed. Kruskal–Wallis ANOVA with Dunn’s multiple comparison correction, sg-Ctrl (n = 847 cells), KO-N9 (n = 124 cells), KO-N13 (n = 375 cells), error bar represents median with interquartile range. g Quantification (left) and representative images (right) of cell invasion for 4T1 (at 48 h) and (h) 6DT1 (at 24 h) NUP210-depleted cells. Two-tailed t test, mean ± s.d. n = 3 biological replicates. Scale bar = 100 μm.