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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: FEBS J. 2021 Jun 30;289(15):4430–4446. doi: 10.1111/febs.16074

Fig. 3. Image analysis techniques for the study of actin dynamics.

Fig. 3.

Spatial and temporal changes in the fluorescence intensity of F-actin markers can be tracked using several techniques as described here. Top panel: Kymography. Slope of fluorescent streaks in the kymograph represents the speed (v) of the moving particle; Middle panel: Correlation-based methods. Color-coded arrows represent local speed (color and length of arrows) and direction of flows; Bottom panel: Optical flow. Color-coded arrows represent directionality of clusters. These methods enable quantification of the speed and directionality of actin flows, allowing a more complete characterization of actin dynamics during B-cell immune synapse formation.