Skip to main content
. 2021 Dec 8;2(4):100977. doi: 10.1016/j.xpro.2021.100977
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

U-13C-15N-cysteine Cambridge Isotope Laboratories Cat# CNLM-3871-H-PK
U-13C-15N-cystine Cambridge Isotope Laboratories Cat# CNLM-4244-H-PK
13C-U-15N-glutathione Cambridge Isotope Laboratories Cat# CNLM-6245-HP-PK
13C-U-15N-glutathione disulfide Cambridge Isotope Laboratories Cat# CNLM-8782-PK
Benzyl choloroformate ACROS ORGANICS Cat# 152945000
0.5M EDTA Lonza Cat# 51234
Acetonitrile HPLC grade Sigma-Aldrich Cat# 439134-1L
Methanol HPLC grade Sigma-Aldrich Cat# 34860-1L-R
Formic Acid Sigma-Aldrich Cat# 33015-500ML
Dialyzed Fetal Bovine Serum Sigma-Aldrich Cat# F0392
KL-11743 Kadmon Corporation N/A
DMEM without glucose Thermo Fisher Scientific Cat# 11966
Penicillin-Streptomycin Life Technologies Cat# 15140-122
DMEM Sigma-Aldrich Cat# D6429
0.25% Trypsin EDTA Life Technologies Cat# 25200056
DPBS Sigma-Aldrich Cat# D8537
Water LC-MS grade Fisher Scientific Cat# W64
Methanol LC-MS grade Fisher Scientific Cat# A456-4
Tributylamine Sigma-Aldrich Cat# 90781
Acetic acid LC-MS grade Fisher Scientific Cat# A11350
Hydrochloric acid Fisher Scientific Cat# A508
Triethylamine Fisher Scientific Cat# AC219510500
Negative Ion Calibration Solution Thermo Fisher Scientific Cat# 88324

Experimental models: Cell lines

UMRC6 Laboratory of W. G. Kaelin Lab N/A

Software and algorithms

GraphPad GraphPad https://www.graphpad.com, RRID:SCR_002798
Xcalibur 3.0.63 Thermo Scientific https://www.thermofisher.com/,
RRID:SCR_014593
El-MAVEN Elucidata https://resources.elucidata.io/elmaven
ProteoWizard SourceForge https://proteowizard.sourceforge.io/index.shtml

Other

Refrigerated micro-centrifuge Eppendorf Cat# EPP-5424R
CO2 Incubator Thermo Scientific Cat# TH-370N
BSC Cabinet NuAire Cat# ES NU-427
Plate Rocker Corning Cat# 6780-FP
PCV Cell Counting Tubes Sigma-Aldrich Cat# Z760986-50EA
Plastic Cell Scrapers Corning Cat# 08-771-1B
Cold Block Thermo Scientific Cat# 88-870-104
Eppendorf tubes Thermo Scientific Cat# MCT-175C
35 mm dish Thermo Scientific Cat# 353001
Synergi Hydro-RP C18 column Phenomenex Cat# 00D-4387-B0
Autosampler vials Thermo Scientific Cat# 03-377-299
Autosampler vial caps Thermo Scientific Cat# 14-823-399