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Step by step quantification of focal adhesion (FA) numbers and lengths
Step 77: Representative image shows merged image of primary mouse astrocytes which co-labeled with vinculin marker (magenta), actin filaments (red), GFAP (green) and for nuclear staining DAPI (blue). Step 78: After splitting channels, only vinculin staining is shown. Step 79: 8-bit image. Step 80: FFT Bandpass filter applied image. Step 81–83: Subtracting background and detecting FAs in the cell. Scale bar: 10 μm.
