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. 2021 Dec 8;2(4):100954. doi: 10.1016/j.xpro.2021.100954

Figure 8.

Figure 8

Representative indirect immunostaining of BL/6 mouse brain primary cell cultures

(A) Indirect immunofluorescence double staining of MAP2 (green) and primary astrocyte marker GFAP (magenta), as common markers for primary neurons and primary astrocytes, counterstained by DAPI (blue) for nuclear DNA, before and after the purification of astrocytes.

(B) Immunofluorescence staining of a primary astrocyte culture after primary astrocyte purification. OPC, microglia and primary astrocytes cells were stained by antibodies against PDGRF-α, CD68 and GFAP, respectively.

(C) Quantification of the number of GFAP, PDGRF-α and CD68-stained positive cells after astrocyte purifications demonstrates that primary astrocytes are the most prominent cell type (∼80%). N = 360–490 cells per condition, 3 independent experiments. Scale bar: 10 μm.